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When you create a new document, start typing, or create a new object, it is based on default settings that you can change. For example, by default, a new document is always letter-sized, but if you design only CD covers, you can change the default.

n Most panels provide a pop-up menu, called a flyout menu, that you can display by clicking the iconic button in the upper-right corner. The flyout menu provides commands related to the current panel s contents, as shown earlier in Figure 2.10.

these cells will detect mRNA upregulation of both glial brillary acidic protein (GFAP) and neuro lament protein. Increases in GFAP and neuro lament proteins can also be detected by Western blotting. Immuno uorescent staining shows cells positive for neuro lament, GFAP, and -tubulin III. Procedures are similar to those discussed above using the primers in Table 11.3.

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Figure 3-9: The Document toolbar offers easy access to an important element of a Web page, the title. The first set of buttons in the Document toolbar is dedicated to the various views: Code, Code and Design, and Design. These buttons are mutually exclusive because only one view can be shown at a time. To the right of the first three buttons is one more view-related button: Live Data view. As previously explained, when Live Data view is enabled, Dreamweaver sends your page to the testing server and incorporates the results in an editable format on the screen. When you switch to Live Data view, another toolbar becomes visible; it is described later in this chapter in the section The Live Data toolbar. Next to the Live Data View button is a text field for displaying and altering the title of your document. Dreamweaver, by default, titles every new page Untitled Document. Not only is it considered bad form to keep this default title, search engines need a relevant title to properly index a site. To change a page title, enter the new text in the Title field and press Enter (Return) to confirm your modification.

1. To create a value list, choose File Define Value Lists. The Define Value Lists dialog appears (see Figure 4-24).

10.2.2. For Isolation of Chondroprogenitor Cells from Human Synovium 10.2.2.1. High-Collagenase Digestion Medium. DMEM supplemented with: 5% FBS Collagenase 1500 U/mL HEPES 10 mM Antibiotic/antimycotic (10,000 U/mL penicillin, 10 mg/mL streptomycin, 25 g/mL amphotericin B) 1% (v/v; 100 U/mL, 100 g/mL, and 0.25 g/mL, respectively, nal concentrations) Gentamicin (50 mg/mL) 0.2% (v/v; 100 g/mL nal) 10.2.2.2. Expansion Medium. DMEM supplemented with: FBS bFGF TGF- 1 Gentamicin, 50 mg/mL 10.2.2.3. Differentiation Medium. DMEM supplemented with: 1% (v/v) ITS (insulin, transferrin, selenium; see Section 10.6) TGF- 1 15 ng/mL HEPES, 1 M 1% (10 mM nal) Gentamicin, 50 mg/mL 0.2% (100 g/mL nal) 10.2.2.4. Phosphate-Buffered Saline. (i) PBSC: Dulbecco s phosphate-buffered saline complete with 1 mM CaCl2 , 1 mM MgCl2 (ii) PBSA: Dulbecco s phosphate-buffered saline without Ca2+ and Mg2+ 20% 5 ng/mL 2.5 ng/mL 0.2% (100 g/mL nal)

(choice | seq) ( | * | + ) (Name | choice | seq) ( | * | + ) ( S cp ( S | S cp ) + S ) ( S cp ( S , S cp) * S )

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(vii) Ascorbic acid-2-phosphate (viii) Dexamethasone (ix) GASP antibiotics (see Section 11.2.1) 11.2.9. HBSS/2FB Hanks BSS with 2% FBS 11.2.10. DMEM/2FB DMEM with 2% FBS 11.2.11. Keratocyte Differentiation Medium Advanced D-MEM (Invitrogen) with: (i) Basic broblast growth factor (FGF-2) (ii) Ascorbic acid-2-phosphate 11.2.12. Chondrocyte Differentiation Medium (CDM) DMEM/MCDB-201, 60:40 with: (i) (ii) (iii) (iv) (v) FBS Ascorbic acid-2-phosphate Dexamethasone TGF- Sodium pyruvate

Value: [disc | circle | square | decimal | lower-roman | upper-roman | lower-alpha | upper-alpha | none] || [inside | outside] || [<url> | none] Initial: Not de ned for shorthand properties Applies to: Elements with display value list-item Inherited: Yes Percentage values: N/A

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