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Symbol < > = Meaning Less than Less than or equal to Greater than Greater than or equal to An exact word match Example <50000 (Find persons who make less than $50,000 per year.) 5 (Find cities with annual rainfall less than or equal to 5 inches.) >7/4/94 (Find any date after July 4, 1994.) 10:43 (Find any time that is equal to or later than 10:43 a.m.) Match exactly a whole word portion of the field s contents. (For example, =Toys will find Toys R Us but won t find Tolstoy.) 80210...80218 (Find ZIP codes that fall within that range.) ! (Entered in an ID field, selects all records with duplicate ID numbers.) // (Selects all records with today s date in the chosen Date field.)

The main goal of successful microcarrier cultivation is to maximize cell density without detrimental effects of agitation, while providing adequate oxygen transfer and a homogeneous bioreactor environment A major problem is that adherent cells can be removed from the microcarrier surfaces at steady laminar stresses in the range 05 to 10 N/m2 accompanied by loss of viable cells and productivity (Aunins and Henzler, 1993) Unlike in suspension cultures at realistic power inputs (Oh et al, 1989, 1992), Croughan and Wang (1987) showed that cell damage in microcarrier cultures could be related to the Kolmogorov scale of turbulent eddy dissipation In microcarrier suspension cultures, turbulent eddies in the viscous dissipation region are often intermediate in size between the cells and microcarriers.

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you first create an HTML file and then replace its entire contents with your own data or nothing at all. The following custom function, developed by Andrew Wooldridge, makes and saves a new, blank text file:

of orbital electrons equals the number of nuclear protons. Although each electron orbits at high speed, it remains in its orbit because the electrical force draws it toward the positively charged nucleus. This attraction keeps the moving electron in its orbit in much the same way as a string tied to a ball will hold it in its path as you swing it rapidly around your head (Fig. 1-6).

Halvorsen, YD, Bond A, Sen A, Franklin DM, Lea-Currie YR, Sujkowski D, Ellis PN, Wilkison WO, Gimble JM (2001b) Thiazolidinediones and glucocorticoids synergistically induce differentiation of human adipose tissue stromal cells: biochemical, cellular, and molecular analysis Metabolism 50:407 413 Hauner, H, G, Entenmann G, Wabitsch M, Gaillard D, Ailhaud G, Negrel R, Pfeiffer EF (1989) Promoting effect of glucocorticoids on the differentiation of human adipocyte precursor cells cultured in a chemically de ned medium J Clin Invest 84(5):1663 1670 Holiday, R (1996) DNA methylation in eukaryotes: 20 years on In Epigenetic mechanisms of gene regulation, AD Riggs, Editor Cold Spring Harbor Laboratory Press: New York p 692 Kang, SK, Lee DH, Bae YC, Kim HK, Baik SY, Jung JS (2003) Improvement of neurological de cits by intracerebral transplantation of human adipose tissue-derived stromal cells after cerebral ischemia in rats Exp Neurol 183:355 366 Kral, J.

In this case, the high rate of speci c energy dissipation as eddies interact with the surface of the microcarriers causes local transient shear rates to be suf ciently high to remove the cells from the microcarrier surface Croughan et al (1989) analyzed selected published data on microcarrier cultivation of FS-4 cells in spinner asks and showed that cell death occurred when the eddy size ( 100 m) calculated using the global energy input to the reactor was smaller than the average microcarrier diameter (185 m) and proposed a model for cell death: dx = dt x ( kd )x for global > dmicrocarrier for global < dmicrocarrier (18-74) (18-75).

and D Crandall (1999) Development of a human adipocyte synthetic polymer scaffold Plast Reconstr Surg 104:1732 1738 Lalikos, JF, Li YQ, Roth TP, Doyle JW, Matory WE, Lawrence WT (1997) Biochemical assessment of cellular damage after adipocyte harvest J Surg Res 70(1):95 100 Moore, JH Jr, Kolaczynski JW, Morales LM, Considine RV, Pietrzkowski Z, Noto PF, Caro JF, et al (1995) Viability of fat obtained by syringe suction lipectomy: effects of local anesthesia with lidocaine Aesthetic Plast Surg 19(4):335 339 Nicoll, S, Wedrychowska A, Smith NR, Bhatnagar RS (2001) Modulation of proteoglycan and collagen pro les in human dermal broblasts by high density micromass culture and treatment with lactic acid suggests change to a chondrogenic phenotype Connect Tissue Res 42(1):59 69 Nnodim, J (1987) Development of adipose tissues Anat Rec 219(4):331 337 Prockop, D (1997) Marrow stromal cells as stem cells for nonhematopoietic tissues Science 276(5309):71 74.

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