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Overexpression of the GST sodB fusion gene is then made possible as follows. A host E. coli strain containing the T7 RNA polymerase is essential for optimal gene expression from the selected pDNA expression vector (pET family). Fortunately, the readily available, attenuated E. coli BL21 strain has just such a chromosomal copy of this heterologous enzyme, and so represents the ideal host for protein expression. Hence, post validation and sequencing of the recombinant pDNA construct, competent E. coli BL21 cells undergo transformation and are then grown on antibiotic selection plates in order to select for recombinant BL21 colonies. Thereafter, selected colonies may be picked, grown on a small scale until saturation (in the presence of antibiotics), then on a larger scale until the A600 absorbance is measured to be approximately 0.6, after which the inducer IPTG is added to induce substantial GST sodB fusion gene transcription. After further cell growth for several hours, samples can be collected and analysed by SDS-PAGE for a protein overexpression band at the appropriate mass (in this case, the mass of a GST SodB fusion protein should be 48 kDa). Assuming that this band can be seen by SDS-PAGE, then protein puri cation can begin (see 2).

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The currently selected date value. Determines whether the current date is highlighted. An array of String values used as labels for the day names. The minimum allowed year. The maximum allowed year. An array of integer values indicating by zero-based index days that aren t selectable. A set of disabled ranges. Each range has named properties of rangeStart and rangeEnd typed as Date values. A selectable range. Requires named properties of rangeStart and rangeEnd typed as Date values.

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You ll need to sign up for an account there, which is a fairly straightforward procedure. After getting yourself logged in, Figure 8-5 shows you what burning a new feed looks like. You can supply a direct feed URL or a blog address from which FeedBurner will attempt to autodetect your feed location. This creates a new proxy feed at FeedBurner through which your original content will be filtered.

Table 2.10 lists the subcribs of computer science for widths 5 N 9. Table 2.11 contains the output of the program Search1, which lists all subcribs of computer science that do not occur in y: Search1

Mobile Station Location Table 4.2. Number of measurements 150 Table 4.3. Number of measurements 200 Table 4.4. Number of measurements 45 E-OTD eld test example (moving MS) Median accuracy 35 m 67% accuracy 42 m 90% accuracy 85 m

2. On the left side of the Search bar, make sure This Mac is selected. 3. On the right side of the Search bar, make sure Contents is selected. 4. In the first criteria row, choose Other in the first pop-up menu to open the Select a

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