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The rst edition of Physical Biochemistry: Principles and Applications set out to describe the physical basis and some examples of applications of key physically-based techniques used in Biochemistry and other areas of molecular life science research In the last decade there has been a noticeable renaissance in some traditional techniques such as X-ray diffraction, ultracentrifugation and electrophoresis in a variety of formats In the same time-frame hyphenated techniques (eg LCtandem MS) have become much more mainstream and some instrumentation has become available in desktop formats making quite sophisticated analysis possible even in the nonspecialist lab The emphasis was on a largely nonmathematical treatment at a level appropriate to students in the penultimate year of a Biochemistry course with a view to making these techniques comprehensible and accessible to a level intermediate between a general Biochemistry textbook and a specialist text.

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The feedback I have had from many readers is that this goal was largely achieved My task with this second edition was to retain as much as possible of the description of physical principles whilst updating and integrating new material into a reasonably compact volume The rst edition was strongly in uenced by the effects of the then-recent completion of the Human and other large-scale genome sequencing projects At the time Proteomics and other -omics technologies were still relatively new paradigms and bioinformatics approaches largely the province of the specialist It is remarkable how quickly these technologies have now become embedded in many areas of biochemical research so that they are now perceived as part of the mainstream In the second edition I have dedicated new chapters to proteomics and bioinformatics, respectively, to re ect this changed situation and to emphasize how interconnected physical and computational techniques have now become.

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The second edition required choices to be made and these are inevitably in uenced by one s perception of what is needed and useful for students to know at the start of their scienti c journey into molecular life science In making these choices I have continued to be guided by what I perceive to be the most generally-used and helpful techniques but I accept that specialists in one or more technique may disagree In preparing this second edition I had help from many of the colleagues listed in the preface to the First Edition In addition, I must thank Dr Rebecca Green, School of Pharmaceutical Sciences, University of Nottingham, UK, for her invaluable comments on surface plasmon resonance ( 3) I am also indebted to the excellent staff at Wiley s especially my editors, Celia Carden and Fiona Woods for their unfailing encouragement and understanding.

However, any errors in the text are my own I earnestly hope that the reader will nd something interesting and thought-provoking in this volume and be encouraged to explore these very powerful approaches in their work Prof David Sheehan University College Cork June 2008.

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The number and type of annotation elements for relationships varies according to the type of relationship. Each of the different relationships is discussed in the following sections.

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1000 Principal stress difference (kPa) 900 800 700 600 500 400 300 200 100 0 0 2 4 6 8 10 12 Axial strain, % 14 16 18 20 2% fiber 1% fiber 0% fiber 3% fiber

(1) L0 (x0 ) = (x x1 )(x x2 ) (x0 x1 )(x0 x2 ) , (x x0 )(x x2 ) (x1 x0 )(x1 x2 ) , (x x0 )(x x1 ) (x2 x0 )(x2 x1 ) , (1) L0 (x) = (1) L1 (x) = (1) L2 (x) = (x x1 )+(x x2 ) (x0 x1 )(x0 x2 ) , (x x0 )+(x x2 ) (x1 x0 )(x1 x2 ) , (x x0 )+(x x1 ) (x2 x0 )(x2 x1 ) .

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