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The complement of this sequence is the sequence of bases that pair with each of the bases listed T pairs with A, and vice versa, and C pairs with G, and vice versa So the complement is TTACGCACCATGGTGCGTAA But notice that this is just the same sequence read backward! Nucleic acid palindromes can be contiguous, as in this example, or can contain intervening sequences that are not part of the palindrome For example, ACGCACCATGCTGTTTGGTGCGT has the palindrome portion of the sequence is shown shaded The intervening sequence, TGCTGTT, is not part of the palindrome Palindrome sequences do occur quite often in nature, at least much more often than one might expect if an organism s nucleotide sequence were entirely random The types of secondary structures that palindromes can form depend on whether the nucleic acid is single stranded or double stranded and on whether the sequence is contiguous or noncontiguous When a palindrome is single stranded and contiguous, a hairpin structure can form One end of the palindrome is complementary to the other, so the nucleotide strand is able to fold back on itself and form base pairs in the region of the palindrome This is illustrated in Fig 10-6a The hairpin region is a double helix even though the nucleic acid is a single strand This is an important point to keep in mind If the palindrome is noncontiguous (ie, it contains an intervening sequence), then when the strand folds back on itself, the conformation is a stem-and-loop structure: a stem where the palindrome bases are self-complementary, and a loop where the intervening sequence is not self-complementary This is shown in Fig 10-6b When a palindrome sequence occurs in a double-stranded DNA, each of the two strands contains its own palindrome One palindrome is the complementary sequence of the other, and both palindromes (by definition) are their own complement when read backward The result is that both strands are able to form either a hairpin or stem-and-loop conformation (a hairpin if the palindrome is contiguous or a stem and loop if not) When both strands form a hairpin or a stem and loop, the resulting structure is called a cruciform See Fig 10-7 Some cruciform structures have been shown experimentally to be binding sites for specific proteins They also can have a significant influence on tertiary structure of DNA.

following table enumerates the steps required. We discuss the term project and other aspects of the application following this table.

Result The Microsoft Development Environment displays with the Start Page shown. Note: This window is illustrated in figure 2.1. You may want to consider closing the Dynamic Help window (by clicking the X in the upper right corner of this window) while using this book. While quite useful in that it provides help related to your current activities, this window also uses quite a bit of CPU and memory resources. The New Project dialog box appears.

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Under Project Types, A list of C# Templates appears. select Visual C# Projects. Under Templates, select Windows Application. In the Name field, enter MyPhotos . Note: The Location entry may vary depending on which version of Windows you are using. To avoid any confusion, this book will use the directory C:\Windows Forms\Projects. In your code, use the default setting provided by the environment.

CREATE THE MYPHOTOS PROJECT (continued) Action 6 Click the OK button. Result The new MyPhotos project is created. The Solution Explorer now contains the files in this solution, and the main window displays a blank form.

As discussed in 6, a manually executed signature, like a photograph, is not globally unique but it has long been recognized by law and custom as a way to confirm one s identity We are regularly asked to put our signature on a variety of documents Putting a person s dynamic signature in a database would make it harder for people to masquerade as someone else with a similar enough face It would also allow for cross comparisons when other checkpoint-related documents are signed (for example, the rental forms for the crop duster) As discussed in 5, the human iris is a highly distinctive identifier Collecting it deliberately involves looking at a camera from a short distance away, which requires cooperation from the subject While somewhat of a new biometric, the iris, because of its inherent distinctiveness and robustness, has tremendous potential as a way individuals can easily establish or confirm their identity Fingerprints have long been recognized to be globally distinctive; they have long been used by law enforcement for identification As explained in s 3 and 17, the FBI has fingerprints (of the ten, rolled variety) on file for over 80 million people already (for example, 40 million in the IAFIS s Criminal Master File as a result of individuals being arrested for felony offenses and 40 million in the largely paper civil files, submission of finger prints being required as a condition of federal government employment, military service, or naturalized citizenship) As explained in detail in the upcoming section, Biometric Data: Freezing Identity, the use of fingerprints is the only established, proven way to identify a person when searching against a large-scale database As discussed in 7, DNA, like fingerprints, is both globally distinctive and apt to be left behind But the deliberate extraction of DNA is intrusive, controversial, and expensive Moreover, at this juncture, no commercially viable DNA-based systems can find a match in near real-time Given the other medical or health-related information that might be inferred from a DNA sample, compelling people to produce DNA will assuredly raise vehement policy and legal objections.

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